Not Released
Experience: Works
Not Used
Get This Part
Generator

Part:BBa_K917001

Designed by: Chris French   Group: iGEM12_Edinburgh   (2012-06-29)

ars promoter plus cscA sucrose assimilation gene

This part consists of the E. coli chromosomal ars promoter and arsR repressor gene (J33201), which together comprise a promoter system induced by sodium arsenate, plus the lacZ' minigene (J33202) and the cscA sucrose hydrolase gene from an E. coli O157 strain (K917000).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1042
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None